Given the high potential of the newly developed FISH techniques and the great hybridization properties of nucleic acid mimics, we propose here to combine these strategies to develop a new color-coded FISH methodology that allows a multiplex (detection of multiple targets simultaneously) and robust:
1. detection of single-copy resistance genes on common and problematic resistant bacteria. This work will allow the recognition of individual genes in less than 3 hours and it will also allow the development of multiplex assays for the detection of different species and resistance mechanisms simultaneously;
2. spatial assessment of gastric microbiota in a mouse model. The establishment of this technique will complement the knowledge on the spatial organization of gastric microbiota, identifying the preferred locations of the microorganisms within the stomach and providing crucial data about the potential function of each member involved in microbiota. This could be could be the key to improve therapeutic strategies to combat or prevent gastric diseases.